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A type I cell-specific protein is a biochemical marker of epithelial injury in a rat model of pneumonia.

Authors: McElroy, MC  Pittet, JF  Hashimoto, S  Allen, L  Wiener-Kronish, JP  Dobbs, LG 
Citation: McElroy MC, etal., Am J Physiol. 1995 Feb;268(2 Pt 1):L181-6.
Pubmed: (View Article at PubMed) PMID:7864138

In this study we determined whether the alveolar fluid content of a specific epithelial type I cell protein, rTI40, can be used as a biochemical marker for lung injury. A model of alveolar epithelial injury was developed by instilling Pseudomonas aeruginosa bacteria (PA103) into the airspaces of anesthetized, ventilated rats. After 6 h, the alveolar fluid content of rTI40 from PA103-treated rats was increased over 80-fold in comparison to alveolar fluid from control rats (P < 0.05). This increase in rTI40 correlated with both morphological evidence of injury to alveolar epithelial type I cells and increased permeability of the alveolar epithelium to protein tracers. In contrast, the lactate dehydrogenase activity of alveolar fluid from PA103-treated rats was elevated only threefold over control values at 6 h (P < 0.05). In a second study using a less injurious strain of P. aeruginosa (PA103 exsA::omega), the alveolar fluid content of rTI40 was the same as control values. These findings indicate that the alveolar fluid content of a type I cell-specific protein can be used as a sensitive and specific biochemical marker of type I cell injury.


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CRRD Object Information
CRRD ID: 2292375
Created: 2008-04-17
Species: All species
Last Modified: 2008-04-17
Status: ACTIVE


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