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Alternative programs of cell death in developing retinal tissue.

Authors: Guimaraes, CA  Benchimol, M  Amarante-Mendes, GP  Linden, R 
Citation: Guimaraes CA, etal., J Biol Chem. 2003 Oct 24;278(43):41938-46. Epub 2003 Aug 12.
Pubmed: (View Article at PubMed) PMID:12917395
DOI: Full-text: DOI:10.1074/jbc.M306547200

We examined cell death in developing retinal tissue, following inhibition of protein synthesis, which kills undifferentiated post-mitotic cells. Ultrastructural features were found of both apoptosis and autophagy. Only approximately half of the degenerating cells were either terminal dUTP nick-end labeling (TUNEL)-positive or reacted with antibodies specific for activated caspases-3 or -9. Bongkrekic acid completely inhibited any appearance of cell death, whereas inhibitors of autophagy, caspases-9 or -3, prevented only TUNEL-positive cell death. Interestingly, inhibition of caspase-6 blocked TUNEL-negative cell death. Simultaneous inhibition of caspases-9 and -6 prevented cell death almost completely, but degeneration dependent on autophagy/caspase-9 still occurred under inhibition of both caspases-3 and -6. Thus, inhibition of protein synthesis induces in the developing retina various post-translational, mitochondria-dependent pathways of cell death. Autophagy precedes sequential activation of caspases-9 and -3, and DNA fragmentation, whereas, in parallel, caspase-6 leads to a TUNEL-negative form of cell death. Additional mechanisms of cell death may be engaged upon selective caspase inhibition.

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CRRD Object Information
CRRD ID: 2301324
Created: 2008-10-06
Species: All species
Last Modified: 2008-10-06
Status: ACTIVE



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RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.