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Differential synthesis of crystallins in the developing rat eye lens.

Authors: Voorter, CE  De Haard-Hoekman, WA  Hermans, MM  Bloemendal, H  De Jong, WW 
Citation: Voorter CE, etal., Exp Eye Res. 1990 Apr;50(4):429-37.
Pubmed: (View Article at PubMed) PMID:2338125

The patterns of protein synthesis in rat lenses ranging in age from newborn to 4 months were compared. After incubation of lenses in [35S]methionine-containing medium it was possible to identify the de novo synthesized crystallins by two-dimensional gel electrophoresis and fluorography, in combination with peptide mapping and immunoblotting. It was found that the relative synthesis of alpha A and beta A3 stays fairly constant in rat lenses of all investigated ages. The relative synthesis of beta B2 and gamma s shows a pronounced increase with age in these post-natal lenses. A differential decrease can be observed in the relative synthesis of the other six gamma-crystallins (gamma A-gamma F). There appears to be a good correlation between the changes in relative synthesis of the various crystallins and previously reported alterations in mRNA levels, although certain mRNAs exhibit marked differences in translational efficiency.


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CRRD Object Information
CRRD ID: 2303725
Created: 2009-02-23
Species: All species
Last Modified: 2009-02-23
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.