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Angiotensin II and NGF differentially influence microtubule proteins in PC12W cells: role of the AT2 receptor.

Authors: Stroth, U  Meffert, S  Gallinat, S  Unger, T 
Citation: Stroth U, etal., Brain Res Mol Brain Res. 1998 Jan;53(1-2):187-95.
Pubmed: (View Article at PubMed) PMID:9473667

Angiotensin AT2 receptors have been shown to play a role in cell differentiation characterized by neurite outgrowth in neuronal cells of different origin. To further investigate AT2 receptor-mediated events leading to neurite formation, we examined the effect of AT2 receptor stimulation on the microtubule components, beta-tubulin, MAP1B and MAP2, by Western blot analysis and immunofluorescence in quiescent and nerve growth factor (NGF)-differentiated PC12W cells. These proteins are involved in neurite extension and neuronal maturation. Whereas NGF (0.5, 10, and 50 ng/ml) up-regulated these proteins after 3 days of stimulation, angiotensin II (ANG II; 10(-7) M) induced a different pattern. In quiescent PC12W cells, AT2 receptor stimulation up-regulated polymerized beta-tubulin and MAP2 but down-regulated MAP1B protein levels. In PC12W cells, differentiated by NGF (0.5 ng/ml), ANG II elevated polymerized beta-tubulin and reduced MAP1B. All ANG II effects were abolished by the AT2 receptor antagonist PD123177 (10(-5) M) but not affected by the AT1 receptor antagonist losartan (10(-5) M). These results implicate a specific role of AT2 receptors in cell differentiation and nerve regeneration via regulation of the cytoskeleton.


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CRRD Object Information
CRRD ID: 2304027
Created: 2009-03-04
Species: All species
Last Modified: 2009-03-04
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.