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Substance P regulates migration in rat intestinal epithelial cells.

Authors: Turner, DJ  Martin, PC  Rao, JN  Greenspon, J  Zou, T  Bass, BL  Wang, JY  Strauch, ED 
Citation: Turner DJ, etal., Ann Surg. 2007 Mar;245(3):408-14.
Pubmed: (View Article at PubMed) PMID:17435548
DOI: Full-text: DOI:10.1097/01.sla.0000245549.57076.db

OBJECTIVE: The current study examined the effect of substance P (SP) upon intestinal epithelial cells and the mechanistic details of this interaction. SUMMARY BACKGROUND DATA: Intestinal epithelial cells must be capable of migration to reseal mucosal wounds for several vital intestinal functions. This process is incompletely understood; however, recent evidence implicates the neurotransmitter SP in this process. METHODS: Normal rat intestinal epithelial cells (IEC-6 cells) were studied to identify the presence of the SP receptor (NK-1 subtype) and then exposed to physiologic doses of SP and antagonists to assess for increased migration. RESULTS: Examination IEC-6 cells revealed the presence of the SP receptor. Wounding of these cells followed by subsequent exposure to SP (10 mol/L) resulted in increased migration. Similarly, SP-induced increases in intracellular calcium concentration and actomyosin stress fiber formation. These effects were all blocked through specific NK-1 receptor antagonists. CONCLUSIONS: These results indicate that SP stimulates intestinal epithelial migration and increases in calcium concentration. These data support a beneficial role for SP in the maintenance of intestinal mucosal homeostasis.

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CRRD Object Information
CRRD ID: 2304338
Created: 2009-03-13
Species: All species
Last Modified: 2009-03-13
Status: ACTIVE



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RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.