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Hydrogen peroxide induces lysosomal protease alterations in PC12 cells.

Authors: Lee, DC  Mason, CW  Goodman, CB  Holder, MS  Kirksey, OW  Womble, TA  Severs, WB  Palm, DE 
Citation: Lee DC, etal., Neurochem Res. 2007 Sep;32(9):1499-510. Epub 2007 Apr 18.
Pubmed: (View Article at PubMed) PMID:17440810
DOI: Full-text: DOI:10.1007/s11064-007-9338-5

Alterations in lysosomal proteases have been implicated in many neurodegenerative diseases. The current study demonstrates a concentration-dependent decrease in PC12 cell viability and transient changes in cystatin C (CYSC), cathepsin B (CATB), cathepsin D (CATD) and caspase-3 following exposure to H2O2. Furthermore, activation of CATD occurred following exposure to H2O2 and cysteine protease suppression, while inhibition of CATD with pepstatin A significantly improved cell viability. Additionally, significant PARP cleavage, suggestive of caspase-3-like activity, was observed following H2O2 exposure, while inhibition of caspase-3 significantly increased cell viability compared to H2O2 administration alone. Collectively, our data suggest that H2O2 induced cell death is regulated at least in part by caspase-3 and CATD. Furthermore, cysteine protease suppression increases CATD expression and activity. These studies provide insight for alternate pathways and potential therapeutic targets of cell death associated with oxidative stress and lysosomal protease alterations.

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CRRD Object Information
CRRD ID: 2306497
Created: 2009-04-20
Species: All species
Last Modified: 2009-04-20
Status: ACTIVE



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RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.