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FGF-2-responsive neural stem cell proliferation requires CCg, a novel autocrine/paracrine cofactor.

Authors: Taupin, P  Ray, J  Fischer, WH  Suhr, ST  Hakansson, K  Grubb, A  Gage, FH 
Citation: Taupin P, etal., Neuron. 2000 Nov;28(2):385-97.
Pubmed: (View Article at PubMed) PMID:11144350

We have purified and characterized a factor, from the conditioned medium of neural stem cell cultures, which is required for fibroblast growth factor 2's (FGF-2) mitogenic activity on neural stem cells. This autocrine/paracrine cofactor is a glycosylated form of cystatin C (CCg), whose N-glycosylation is required for its activity. We further demonstrated that, both in vitro and in vivo, neural stem cells undergoing cell division are immunopositive for cystatin C. Finally, we showed in vivo functional activity of CCg by demonstrating that the combined delivery of FGF-2 and CCg to the adult dentate gyrus stimulated neurogenesis. We propose that the process of neurogenesis is controlled by the cooperation between trophic factors and autocrine/paracrine cofactors, of which CCg is a prototype.


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CRRD Object Information
CRRD ID: 2306506
Created: 2009-04-20
Species: All species
Last Modified: 2009-04-20
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.