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Interleukin-6 Activates Arginine-Vasopressin Neurons in the Supraoptic Nucleus during Immune Challenge in Rats.

Authors: Palin, K  Moreau, ML  Sauvant, J  Orcel, H  Nadjar, A  Duvoid-Guillou, A  Dudit, J  Rabie, A  Moos, F 
Citation: Palin K, etal., Am J Physiol Endocrinol Metab. 2009 Mar 3.
Pubmed: (View Article at PubMed) PMID:19258490
DOI: Full-text: DOI:10.1152/ajpendo.90489.2008

The increase of plasma Arginin-Vasopressin (AVP) release, which translates hypothalamic AVP neuron activation in response to immune challenge, appears to occur independently of plasma osmolality or blood pressure changes. Many studies have shown that major inflammatory mediators produced in response to peripheral inflammation, such as prostaglandin (PG)-E2 and interleukin (IL)-1beta, excite AVP neurons. However, in vivo electrical activation of AVP neurons was still not assessed in relation to plasma AVP release, osmolality, blood pressure or to the expression and role of inflammatory molecules, like PG-E2, IL-1beta, IL-6 and tumor necrosis factor (TNF)-alpha. This study aims at elucidating which factors underlie the activation of AVP neurons in response to immune stimulation mimicked by an intraperitoneal injection of lipopolysaccharide (LPS) in male Wistar rats. LPS treatment concomittanlty decreased diuresis and increased plasma AVP as well as AVP neuron activity in vivo, and these effects occurred as early as 30 min. Activation was sustained for more than six hours. Plasma osmolality did not change while blood pressure only transiently increased during the first hour post-LPS. PG-E2, IL-1beta and TNF-alpha mRNA expression raised 3 h after LPS, while IL-6 mRNA level increased 30 min post-LPS. In vivo electrophysiological recordings showed that brain IL-6 injection increased AVP neuron activity similarly to peripheral LPS treatment. In contrast, brain injection of anti-IL-6 antibodies prevented the LPS induced-activation of AVP neurons. Taken together, these results suggest that the early activation of AVP neurons in response to LPS injection is induced by brain IL-6. Key words: Lipopolysaccharide induced-hypothalamic vasopressin neuron activation, brain cytokines, plasma osmolality, diuresis.


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CRRD Object Information
CRRD ID: 2306936
Created: 2009-05-12
Species: All species
Last Modified: 2009-05-12
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.