Increased expression of interleukin-1alpha and interleukin-1beta is associated with experimental varicocele.

Authors: Sahin, Z  Celik-Ozenci, C  Akkoyunlu, G  Korgun, ET  Acar, N  Erdogru, T  Demir, R  Ustunel, I 
Citation: Sahin Z, etal., Fertil Steril. 2006 Apr;85 Suppl 1:1265-75.
Pubmed: (View Article at PubMed) PMID:16616101
DOI: Full-text: DOI:10.1016/j.fertnstert.2005.10.025

OBJECTIVE: To describe the effect of varicocele, in an experimental rat model, on the levels of IL-1alpha and IL-1beta proteins in testis tissue. DESIGN: Comparative and controlled study. SETTING: Experimental research. ANIMAL(S): Wistar male rats in experimental and control groups. INTERVENTION(S): The control group underwent sham operation (n = 6). Experimental groups underwent partial ligation of the renal vein to induce experimental varicocele and were then killed at 9 (n = 6), 11 (n = 6), and 13 (n = 6) weeks after induction of varicocele. MAIN OUTCOME MEASURE(S): Histologic evaluation of the varicocele model was determined by periodic acid-Schiff staining of paraffin-embeded testicular tissues. Levels of cytokines were assessed by immunohistochemistry and Western blot analysis. RESULT(S): Varicocele caused testicular damage, especially in 11- and 13-week-old varicocele groups. In sham-operated rats, Golgi complexes of round spermatids expressed especially the alpha form of IL-1. By the progression of varicocele, the IL-1alpha expression increased temporally in Sertoli cells, spermatogonia, primary spermatocytes, spermatids, and Leydig cells. The expression of IL-1beta was seen in Leydig cells in sham-operated rats. The IL-1beta expression was also increased upon progression of varicocele in Leydig cells, Sertoli cells, and spermatogonia. CONCLUSION(S): We suggest that IL-1alpha and IL-1beta are the regulators of testicular function. Certain pathologic conditions, e.g., varicocele, cause an increase in the expressions of such proinflammatory cytokines. The increased expression of IL-1alpha and IL-1beta in varicocele shifts the balance in favor of inflammatory and immune responses and causes detrimental effects in testis tissue, which may cause male infertility.


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CRRD Object Information
CRRD ID: 2311094
Created: 2009-06-23
Species: All species
Last Modified: 2009-06-23
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.