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Synaptotagmin IX regulates Ca2+-dependent secretion in PC12 cells.

Authors: Fukuda, M  Kowalchyk, JA  Zhang, X  Martin, TF  Mikoshiba, K 
Citation: Fukuda M, etal., J Biol Chem. 2002 Feb 15;277(7):4601-4. Epub 2001 Dec 21.
Pubmed: (View Article at PubMed) PMID:11751925
DOI: Full-text: DOI:10.1074/jbc.C100588200

Synaptotagmin (Syt) I-deficient phaeochromocytoma (PC12) cell lines show normal Ca(2+)-dependent norepinephrine (NE) release (Shoji-Kasai, Y., Yoshida, A., Sato, K., Hoshino, T., Ogura, A., Kondo, S., Fujimoto, Y., Kuwahara, R., Kato, R., and Takahashi, M. (1992) Science 256, 1821-1823). To identify an alternative Ca(2+) sensor, we searched for other Syt isoforms in Syt I-deficient PC12 cells and identified Syt IX, an isoform closely related to Syt I, as an abundantly expressed dense-core vesicle protein. Here we show that Syt IX is required for the Ca(2+)-dependent release of NE from PC12 cells. Antibodies directed against the C2A domain of either Syt IX or Syt I inhibited Ca(2+)-dependent NE release in permeable PC12 cells indicating that both Syt proteins function in dense-core vesicle exocytosis. Our results support the idea that Syt family proteins that co-reside on secretory vesicles may function cooperatively and redundantly as potential Ca(2+) sensors for exocytosis.

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CRRD Object Information
CRRD ID: 2311146
Created: 2009-06-26
Species: All species
Last Modified: 2009-06-26
Status: ACTIVE



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RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.