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Adenovirus-transferred HNF-3 gamma conserves some liver functions in primary cultured hepatocytes of adult rats.

Authors: Nakamura, T  Mura, T  Saito, K  Ohsawa, T  Akiyoshi, H  Sato, K 
Citation: Nakamura T, etal., Biochem Biophys Res Commun. 1998 Dec 18;253(2):352-7.
Pubmed: (View Article at PubMed) PMID:9878541
DOI: Full-text: DOI:10.1006/bbrc.1998.9797

Hepatocyte nuclear factor-3 (HNF-3) isoforms are key factors for regulation of gene expression and differentiation in hepatocytes. HNF-3gamma is abundantly expressed in the mature liver, but down-regulated in primary cultured hepatocytes, in which some other hepatic gene expressions are also decreased. In this study, the primary hepatocytes were infected with the recombinant adenovirus carrying HNF-3gamma gene (AxCAHNF3gamma), and this led to marked induction of the HNF-3gamma gene. As a result, the expressions of albumin, catalase, and ornithine transcarbamylase (OTC) genes were also recovered to significant levels in the AxCAHNF3gamma-infected hepatocytes. Moreover, hepatocyte proliferation stimulated by epidermal growth factor (EGF) and insulin was also inhibited by AxCAHNF3gamma infection. Our results demonstrate that the enforced expression of HNF-3gamma gene can lead to conservation of some original liver functions in the primary cultured hepatocytes accompanied by morphological differentiation and growth inhibition.

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CRRD Object Information
CRRD ID: 2315086
Created: 2009-12-17
Species: All species
Last Modified: 2009-12-17
Status: ACTIVE



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