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VIP provides cellular protection through a specific splice variant of the PACAP receptor: a new neuroprotection target.

Authors: Pilzer, I  Gozes, I 
Citation: Pilzer I and Gozes I, Peptides. 2006 Nov;27(11):2867-76. Epub 2006 Aug 14.
Pubmed: (View Article at PubMed) PMID:16905223
DOI: Full-text: DOI:10.1016/j.peptides.2006.06.007

Vasoactive intestinal peptide (VIP) was known to provide neuroprotection. Three VIP receptors have been cloned: VPAC1, VPAC2 and PAC1. A specific splice variant of PAC1 in the third cytoplasmatic loop, hop2, was implicated in VIP-related neuroprotection. We aimed to clone the hop2 splice variant, examine its affinity to VIP and investigate whether it mediates the VIP-related neuroprotective activity. The PAC1 cDNA was cloned from rat cerebral astrocytes. Using genetic manipulation the hop2 splice variant was obtained, then inserted into an expression vector and transfected into COS-7 cells that were used for binding assays. Results showed that VIP bound the cloned hop2 splice variant. Stearyl-neurotensin(6-11) VIP(7-28) (SNH), an antagonist for VIP, was also found to bind hop2. In addition, VIP protected COS-7 cells expressing hop2 from oxidative stress. Parallel assays demonstrated that VIP increased cAMP accumulation in COS-7 cells expressing hop2. These results support the hypothesis that hop2 mediates the cytoprotective effects attributed to VIP.


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CRRD Object Information
CRRD ID: 2315974
Created: 2010-01-20
Species: All species
Last Modified: 2010-01-20
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.