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Axonal guidance protein FEZ1 associates with tubulin and kinesin motor protein to transport mitochondria in neurites of NGF-stimulated PC12 cells.

Authors: Fujita, T  Maturana, AD  Ikuta, J  Hamada, J  Walchli, S  Suzuki, T  Sawa, H  Wooten, MW  Okajima, T  Tatematsu, K  Tanizawa, K  Kuroda, S 
Citation: Fujita T, etal., Biochem Biophys Res Commun. 2007 Sep 28;361(3):605-10. Epub 2007 Jul 23.
Pubmed: (View Article at PubMed) PMID:17669366
DOI: Full-text: DOI:10.1016/j.bbrc.2007.07.050

Fasciculation and elongation protein zeta-1 (FEZ1) promotes efficiently the neurite elongation of rat phaeochromocytoma PC12 cells. We here characterized FEZ1 in PC12 cells. Nerve growth factor (NGF) stimulation induces significant expression of endogenous FEZ1 in PC12 cells. Upon NGF stimulation FEZ1 localizes in both cytoplasm and neuritis, co-localizing with mitochondria. Silencing of FEZ1 by RNA interference efficiently reduces NGF-induced neurite elongation and the anterograde motility of mitochondria in PC12 cells. Immunoprecipitation and pulldown assay shows that FEZ1 interacts with kinesin superfamily protein 5 (KIF5) and tubulin. Thus, our results suggest that the FEZ1/kinesin complex functions for the transport of mitochondria along microtubules toward the extending neurites in differentiating PC12 cells.

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CRRD Object Information
CRRD ID: 2316312
Created: 2010-02-05
Species: All species
Last Modified: 2010-02-05
Status: ACTIVE



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RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.