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Involvement of an endogenous sialidase in skeletal muscle cell differentiation.

Authors: Sato, K  Miyagi, T 
Citation: Sato K and Miyagi T, Biochem Biophys Res Commun. 1996 Apr 25;221(3):826-30.
Pubmed: (View Article at PubMed) PMID:8630046
DOI: Full-text: DOI:10.1006/bbrc.1996.0681

We have previously observed that the cytosolic sialidase gene is highly expressed in rat skeletal muscle and that it contains an enhancer/promoter region which is transcriptionally active in rat L6 myogenic cells. Here we present evidence for the involvement of cytosolic sialidase in myoblast differentiation. During L6 myoblast differentiation induced by serum depletion, cytosolic sialidase is increased in activity as well as in terms of mRNA level. Sialidase activity is essentially lacking in untreated myoblasts but appears concomitantly with myotube formation after induction of differentiation. The mRNA becomes able to be detected after 3 days at the time which myogenin mRNA reaches a maximum level. Myotube formation can, in fact, be blocked by the addition of an antisense oligodeoxyribonucleotide complementary to the first 8 codons of the cytosolic sialidase.


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CRRD Object Information
CRRD ID: 2316563
Created: 2010-02-16
Species: All species
Last Modified: 2010-02-16
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.