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A subset of p23 localized on secretory granules in pancreatic beta-cells.

Authors: Hosaka, M  Watanabe, T  Yamauchi, Y  Sakai, Y  Suda, M  Mizutani, S  Takeuchi, T  Isobe, T  Izumi, T 
Citation: Hosaka M, etal., J Histochem Cytochem. 2007 Mar;55(3):235-45. Epub 2006 Nov 13.
Pubmed: (View Article at PubMed) PMID:17101722
DOI: Full-text: DOI:10.1369/jhc.6A7093.2006

Proteins on the membrane of secretory granules (SGs) involved in their biogenesis and exocytosis are poorly characterized compared with those of synaptic vesicle in neurons. Thus the secretory granule membrane was prepared from a mouse pancreatic beta-cell line MIN6 by subcellular fractionation, and protein constituents were analyzed by microscale two-dimensional liquid chromatography coupled with electrospray ionization tandem mass spectrometry. Using this proteomics approach, one of the p24 family proteins, p23, was unexpectedly found in the granule fraction, although p24 proteins are generally regarded as functioning in the early secretory pathways between the endoplasmic reticulum and the Golgi apparatus. We further showed that p23 is expressed at high levels in endocrine cells. Furthermore, immunocytochemical analyses of pancreatic beta-cells at the light and electron microscopic levels demonstrated that a significant amount of p23 is localized on the insulin granule membrane, although it is most intensely concentrated at the cis-Golgi compartment as previously shown in non-endocrine cells. These findings suggest that a fraction of p23 enters post-Golgi compartments and may function in the biogenesis and/or quality control of SGs.


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CRRD Object Information
CRRD ID: 2317246
Created: 2010-03-23
Species: All species
Last Modified: 2010-03-23
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.