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Effects of 5-fluorouracil derivative UFT on thymidylate synthetase and thymidine kinase in rat colorectal tumors.

Authors: Sakamoto, S  Kawachi, Y  Iwama, T  Kuwa, K  Suzuki, T  Mitamura, T  Kudo, H  Sassa, S  Yoshimura, S  Maemura, T  Nakayama, T  Ohsawa, M  Hara, Y 
Citation: Sakamoto S, etal., Anticancer Res. 1999 Jan-Feb;19(1A):245-50.
Pubmed: (View Article at PubMed) PMID:10226549

BACKGROUND: Thymidylate synthetase and thymidine kinase are key enzymes involved in de novo and salvage pathways for pyrimidine nucleotide synthesis, respectively. MATERIALS AND METHODS: Weekly injections of 1,2-dimethyl-hydrazine induced high incidence of colorectal adenocarcinomas in rats. RESULTS: An increased activity of thymidylate synthetase was found in the poorly differentiated adenocarcinomas of the chemically induced rat colorectal tumors. Six-week oral administration of 1-(2-tetrahydrofuryl)-5-fluorouracil in combination with uracil (UFT) reduced the total number of colorectal tumors, with the reduction of thymidylate synthetase activity in the poorly-differentiated type, though the mRNA expression of thymidylate synthetase and thymidine kinase differed little between the groups with or without UFT treatment. CONCLUSIONS: These results indicate that the long-term oral administration using UFT suppresses colorectal carcinogenesis and the growth of the poorly-differentiated type tumors.


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CRRD Object Information
CRRD ID: 2317418
Created: 2010-04-05
Species: All species
Last Modified: 2010-04-05
Status: ACTIVE


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