Reelin is a positional signal for the lamination of dentate granule cells.

Authors: Zhao, S  Chai, X  Forster, E  Frotscher, M 
Citation: Zhao S, etal., Development. 2004 Oct;131(20):5117-25.
Pubmed: (View Article at PubMed) PMID:15459104
DOI: Full-text: DOI:10.1242/dev.01387

Reelin is required for the proper positioning of neurons in the cerebral cortex. In the reeler mutant lacking reelin, the granule cells of the dentate gyrus fail to form a regular, densely packed cell layer. Recent evidence suggests that this defect is due to the malformation of radial glial processes required for granule cell migration. Here, we show that recombinant reelin in the medium significantly increases the length of GFAP-positive radial glial fibers in slice cultures of reeler hippocampus, but does not rescue either radial glial fiber orientation or granule cell lamination. However, rescue of radial glial fiber orientation and granule cell lamination was achieved when reelin was present in the normotopic position provided by wild-type co-culture, an effect that is blocked by the CR-50 antibody against reelin. These results indicate a dual function of reelin in the dentate gyrus, as a differentiation factor for radial glial cells and as a positional cue for radial fiber orientation and granule cell migration.

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CRRD ID: 2317799
Created: 2010-04-26
Species: All species
Last Modified: 2010-04-26
Status: ACTIVE



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RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.