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[In vivo effect of annexin I down-regulation on the growth of human pancreatic cancer in nude mice]

Authors: Liu, Q  Hu, H  Ran, YL  Wang, CF  Zhao, DB  Tian, YT  Sun, LX  Xie, YB  Yang, ZH  Zhao, P 
Citation: Liu Q, etal., Zhonghua Zhong Liu Za Zhi. 2008 Dec;30(12):897-900.
Pubmed: (View Article at PubMed) PMID:19173988

OBJECTIVE: To further explore the effect of annexin I on the tumor growth of human pancreatic cancer in nude mice. METHODS: To knock down the expression of annexin I in pancreatic carcinoma cells by RNAi. A nude mouse model of human pancreatic cancer was established by subcutaneous inoculation of human pancreatic cancer cell line Suit-II cells. The effect of annexin I on tumor growth was assessed by tumor growth curve and tumor weight records, and Westen blot and flow cytometry were used to examine the expression of annexin I after annexin I-knocking down. RESULTS: The results of Western blot revealed that the expression of annexin I was significantly decreased in Suit-II cells transfected with pSilencer-annexin I-siRNA1, and almost completely inhibited in the cells transfected with pSilencer-annexin I-siRNA2 and pSilencer-annexin I-siRNA3. The growth of tumors transfected with annexin I-siRNA2 and annexin I-siRNA3 was inhibited by 76.6% and 68.4%, respectively, in comparison with that of tumor from the parent Suit-II cells. At 44 days after tumor cell inoculation, the tumor weight was 0.8987 g (transfected with annexin I-siRNA2) and 0.8992 g (transfected with annexin I-siRNA3), significantly lower (P < 0.001) than that of tumor from parent Suit-II cells (2.5866 g) and transfected with annexin I-siRNAN (2.4070 g). CONCLUSION: annexin I promotes the growth and proliferation of pancreatic carcinoma cells in vivo and increases the ability of tumor formation in nude mice. The results of this study support that annexin I may become a potential target in gene therapy for this disease.


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CRRD Object Information
CRRD ID: 2325722
Created: 2010-06-07
Species: All species
Last Modified: 2010-06-07
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.