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Expression of pancreatic endocrine markers by prolactin-treated rat bone marrow mesenchymal stem cells.

Authors: Gonzalez, P  Santos, TM  Calil, A  Corradi Perini, C  Percegona, LS  Silva, IC  Kuligovski, C  Aguiar, AM  Camara, NO  Aita, CA 
Citation: Gonzalez P, etal., Transplant Proc. 2010 Mar;42(2):566-9.
Pubmed: (View Article at PubMed) PMID:20304194
DOI: Full-text: DOI:10.1016/j.transproceed.2010.01.031

BACKGROUND: Mesenchymal stem cells (MSCs) are an attractive source for generation of cells with beta-cell properties. Previous studies have demonstrated the ability of prolactin to induce an increase in beta-cell mass and maturation, which suggests beneficial effects of its use in MSC differentiation protocols. OBJECTIVE: To evaluate the expression of endocrine differentiation markers in rat MSCs treated in vitro with prolactin. METHODS: Mesenchymal stem cells from bone marrow of Wistar rats were isolated, expanded, and characterized. Differentiation of MSCs was induced in medium containing 23 mmol/L of glucose, and nicotinamide, 2-mercaptoethanol, and exendin-4, in the presence or absence of 500 ng/mL of rat recombinant prolactin. Expression of endocrine markers and prolactin receptor genes was evaluated using real-time polymerase chain reaction, and compared between culture stages and presence vs absence of prolactin in the culture medium. Expression of insulin, somatostatin, glucagon, and pancreatic and duodenal homeobox 1 was also evaluated at immunofluorescence microscopy. RESULTS: Isolated cells were mostly MSCs, as confirmed at fluorescent-activated cell sorting and cytochemistry. Pax6, Ngn-3, Isl1, NeuroD1, Nkx2.2, and Nkx6.1 exhibited varied expression during culture stages. The long form of the prolactin receptor messenger RNA was induced in prolactin-treated cultures (P < .05). The somatostatin gene was induced in early stages of differentiation (P < .05), and its expression was induced by prolactin, as confirmed using immunofluorescence. CONCLUSION: Culture of rat bone marrow MSCs in differentiation medium induces expression of pancreatic endocrine-specific genes, and somatostatin and prolactin receptor expression was also induced by prolactin.

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CRRD Object Information
CRRD ID: 2326021
Created: 2010-06-17
Species: All species
Last Modified: 2010-06-17
Status: ACTIVE



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RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.