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Regulation of dendrite morphology and excitatory synapse formation by zDHHC15.

Authors: Shah, Bhavin S  Shimell, Jordan J  Bamji, Shernaz X 
Citation: Shah BS, etal., J Cell Sci. 2019 Jul 5;132(13). pii: jcs.230052. doi: 10.1242/jcs.230052.
Pubmed: (View Article at PubMed) PMID:31189538
DOI: Full-text: DOI:10.1242/jcs.230052

Protein palmitoylation is the most common post-translational lipid modification in the brain and is mediated by a family of 24 zDHHC enzymes. There has been growing interest in zDHHCs due to mounting evidence that these enzymes play key roles in the development and function of neuronal connections, and the fact that a number of zDHHCs have been associated with neurodevelopmental and neurodegenerative diseases. Loss-of-function variants in several zDHHCs, including zDHHC15, have been identified in patients with intellectual disabilities; however, the function of zDHHC15 in the brain has not been well studied. Here, we demonstrate that knocking down zDHHC15 in primary rat hippocampal cultures reduces dendritic outgrowth and arborization, as well as spine maturation. Moreover, knockdown of zDHHC15 reduces palmitoylation of PSD-95 and its trafficking into dendrites, resulting in an overall decrease in the density of excitatory synapses being formed onto mutant cells.

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CRRD Object Information
CRRD ID: 25671406
Created: 2020-04-25
Species: All species
Last Modified: 2020-04-25
Status: ACTIVE



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