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Cationic amino acid transporter 1-mediated L-arginine transport at the inner blood-retinal barrier.

Authors: Tomi, Masatoshi  Kitade, Naohisa  Hirose, Shirou  Yokota, Noriko  Akanuma, Shin-Ichi  Tachikawa, Masanori  Hosoya, Ken-ichi 
Citation: Tomi M, etal., J Neurochem. 2009 Nov;111(3):716-25. doi: 10.1111/j.1471-4159.2009.06367.x. Epub 2009 Aug 27.
Pubmed: (View Article at PubMed) PMID:19712052
DOI: Full-text: DOI:10.1111/j.1471-4159.2009.06367.x

The purpose of this study was to identify the transporter mediating l-arginine transport at the inner blood-retinal barrier (BRB). The apparent uptake clearance of [(3)H]L-arginine into the rat retina was found to be 118 microL/(min.g retina), supporting a carrier-mediated influx transport of L-arginine at the BRB. [(3)H]L-arginine uptake by a conditionally immortalized rat retinal capillary endothelial cell line (TR-iBRB2 cells), used as an in vitro model of the inner BRB, was primarily an Na(+)-independent and saturable process with Michaelis-Menten constants of 11.2 microM and 530 microM. This process was inhibited by rat cationic amino acid transporter (CAT) 1-specific small interfering RNA as well as substrates of CATs, L-arginine, L-lysine, and L-ornithine. The expression of cationic amino acid transporter (CAT) 1 mRNA was 25.9- and 796-fold greater than that of CAT3 in TR-iBRB2 and magnetically isolated rat retinal vascular endothelial cells, respectively. The expression of CAT1 protein was detected in TR-iBRB2 cells and immunostaining of CAT1 was observed along the rat retinal capillaries. In conclusion, CAT1 is localized in retinal capillary endothelial cells and at least in part mediates L-arginine transport at the inner BRB. This process seems to be closely involved in visual functions by supplying precursors of biologically important molecules like nitric oxide in the neural retina.


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CRRD Object Information
CRRD ID: 30309907
Created: 2020-06-20
Species: All species
Last Modified: 2020-06-20
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.