Canonical Wnt3a modulates intracellular calcium and enhances excitatory neurotransmission in hippocampal neurons.

Authors: Avila, ME  Sepulveda, FJ  Burgos, CF  Moraga-Cid, G  Parodi, J  Moon, RT  Aguayo, LG  Opazo, C  De Ferrari, GV 
Citation: Avila ME, etal., J Biol Chem. 2010 Jun 11;285(24):18939-47. Epub 2010 Apr 19.
Pubmed: (View Article at PubMed) PMID:20404321
DOI: Full-text: DOI:10.1074/jbc.M110.103028

A role for Wnt signal transduction in the development and maintenance of brain structures is widely acknowledged. Recent studies have suggested that Wnt signaling may be essential for synaptic plasticity and neurotransmission. However, the direct effect of a Wnt protein on synaptic transmission had not been demonstrated. Here we show that nanomolar concentrations of purified Wnt3a protein rapidly increase the frequency of miniature excitatory synaptic currents in embryonic rat hippocampal neurons through a mechanism involving a fast influx of calcium from the extracellular space, induction of post-translational modifications on the machinery involved in vesicle exocytosis in the presynaptic terminal leading to spontaneous Ca(2+) transients. Our results identify the Wnt3a protein and a member of its complex receptor at the membrane, the low density lipoprotein receptor-related protein 6 (LRP6) coreceptor, as key molecules in neurotransmission modulation and suggest cross-talk between canonical and Wnt/Ca(2+) signaling in central neurons.

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CRRD Object Information
CRRD ID: 4110125
Created: 2010-08-17
Species: All species
Last Modified: 2010-08-17
Status: ACTIVE



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RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.