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Recombinant rat surfactant-associated protein D inhibits human T lymphocyte proliferation and IL-2 production.

Authors: Borron, PJ  Crouch, EC  Lewis, JF  Wright, JR  Possmayer, F  Fraher, LJ 
Citation: Borron PJ, etal., J Immunol. 1998 Nov 1;161(9):4599-603.
Pubmed: (View Article at PubMed) PMID:9794387

Components of the airspace-lining material may contribute to the local regulation of immune function within the lung. We report here that recombinant rat pulmonary surfactant-associated protein D (SP-D) inhibits the lectin- and anti-CD3-stimulated proliferation of human PBMCs. Inhibition was associated with a decreased production of IL-2, and the addition of human rIL-2 blocked the inhibitory action of SP-D. These effects were not inhibited by maltose, indicating that the inhibitory activity was not dependent upon the lectin activity of SP-D. Studies employing mutant SP-D lacking N-linked sugars or defective in multimerization further indicated that inhibition was not dependent upon cellular interactions with the N-linked oligosaccharide on SP-D or the oligomerization of trimeric SP-D subunits. Although a peptide containing an inverted DGR showed similar IL-2-dependent effects on anti-CD3-stimulated proliferation, deletion of the conserved DGRDGR sequence near the amino-terminal end of the collagen domain did not decrease the suppressive activity of SP-D. We hypothesize that SP-D can dampen lymphocyte responses to exogenous stimuli and protect the lung against collateral immune-mediated damage.


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CRRD Object Information
CRRD ID: 4144057
Created: 2010-09-30
Species: All species
Last Modified: 2010-09-30
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.