Immunolocalization of androgen receptor and steroidogenic enzymes in cumuli oophori of pre- and post-ovulatory rats.

Authors: Szoltys, M  Slomczynska, M  Knapczyk-Stwora, K  Durlej, M  Lechowska, A  Starowicz, A 
Citation: Szoltys M, etal., Acta Histochem. 2010 Nov;112(6):576-82. Epub 2009 Sep 22.
Pubmed: (View Article at PubMed) PMID:19775733
DOI: Full-text: DOI:10.1016/j.acthis.2009.06.003

Immunolocalization of 3beta-hydroxysteroid dehydrogense (3beta-HSD), cytochrome P450c17 (P450c17) and androgen receptor (AR) were investigated in rat cumuli oophori (COCs) of late pre-ovulatory follicles and in post-ovulatory COCs bearing fertilized oocytes. A gradient of intensity of 3beta-HSD immunolabelling was observed in the granulosa layer of pre-ovulatory follicles, with almost negative immunolabelling in COCs and with the strongest immunoreaction in the mural granulosa cells. Post-ovulatory COCs showed strong 3beta-HSD immunolabelling in the peripheral regions and weak labelling near the oocyte, suggestive of responsiveness of cumulus cells to an anti-luteinizing effect exerted by the fertilized oocyte. In pre-ovulatory follicles, a weak P450c17 immunopositivity was limited to expanded cumulus granulosa cells and the positive labelling persisted in post-ovulatory COCs. P450c17 immunopositivity was also found in ampullary epithelial cells. A strong AR immunopositivity was confined mainly to the COCs in pre-ovulatory follicles and a similar immunoreaction was present in the granulosa cells of ovulated COCs. Simultaneous AR and cytochrome P450c17 immunolabelling in the pre- and post-ovulatory COCs is suggestive of an intra- and paracrine androgen regulation of the cumulus granulosa cell function.

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CRRD Object Information
CRRD ID: 4888508
Created: 2010-11-19
Species: All species
Last Modified: 2010-11-19
Status: ACTIVE



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RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.