Submit Data |  Help |  Video Tutorials |  News |  Publications |  FTP Download |  REST API |  Citing RGD |  Contact   

Tenascin-C deficiency attenuates TGF-{beta}-mediated fibrosis following murine lung injury.

Authors: Carey, WA  Taylor, GD  Dean, WB  Bristow, JD 
Citation: Carey WA, etal., Am J Physiol Lung Cell Mol Physiol. 2010 Sep 10.
Pubmed: (View Article at PubMed) PMID:20833777
DOI: Full-text: DOI:10.1152/ajplung.00385.2009

Tenascin-C (TNC) is an extracellular matrix glycoprotein of unknown function that is highly expressed in adult lung parenchyma following acute lung injury (ALI). Here we report that mice lacking TNC are protected from interstitial fibrosis in the bleomycin model of ALI. Three weeks after exposure to bleomycin, TNC-null mice had accumulated 85% less lung collagen than wildtype mice. The lung interstitium of TNC-null mice also appeared to contain fewer myofibroblasts and fewer cells with intranuclear Smad-2/3 staining, suggesting impaired TGF-beta activation or signaling. In vitro, TNC-null lung fibroblasts exposed to constitutively active TGF-beta expressed less alpha-smooth muscle actin and deposited less collagen I into the matrix than wildtype cells. Impaired TGF-beta responsiveness was correlated with dramatically reduced Smad-3 protein levels and diminished nuclear translocation of Smad-2 and Smad-3 in TGF-beta-exposed TNC-null cells. Reduced Smad-3 in TNC-null cells reflects both decreased transcript abundance and enhanced ubiquitin-proteasome mediated protein degradation. Together, these studies suggest that TNC is essential for maximal TGF-beta action after ALI. The clearance of TNC that normally follows ALI may restrain TGF-beta action during lung healing, while prolonged or exaggerated TNC expression may facilitate TGF-beta action and fibrosis after ALI.

Annotation

Disease Annotations
Objects Annotated

Additional Information

 
CRRD Object Information
CRRD ID: 4889561
Created: 2010-12-06
Species: All species
Last Modified: 2010-12-06
Status: ACTIVE



NHLBI Logo

RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.