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Human monoclonal antibodies to domain C of tenascin-C selectively target solid tumors in vivo.

Authors: Silacci, M  Brack, SS  Spath, N  Buck, A  Hillinger, S  Arni, S  Weder, W  Zardi, L  Neri, D 
Citation: Silacci M, etal., Protein Eng Des Sel. 2006 Oct;19(10):471-8. Epub 2006 Aug 22.
Pubmed: (View Article at PubMed) PMID:16928692
DOI: Full-text: DOI:10.1093/protein/gzl033

We had previously reported that splice isoforms of tenascin-C containing the extra-domain C are virtually absent in normal adult tissues but are highly abundant in high-grade astrocytomas, with a prominent peri-vascular pattern of expression. We now report that the extra-domain C of tenascin-C is strongly expressed in the majority of lung cancers, with a vascular and stromal pattern of expression. Using antibody phage technology, we have generated a human monoclonal antibody (G11), with a dissociation constant K(D) = 4.2 nM for the human domain C. The G11 antibody, expressed in scFv and in mini-antibody (SIP) format, as well as a scFv-interleukin-2 fusion protein, was then characterized in quantitative biodistribution studies using mice grafted subcutaneously with U87 gliomas, revealing a selective tumor uptake, with tumor/blood ratios up to 11.8:1 at 24 h. A radioiodinated preparation of SIP(G11) was also investigated in a double tracer study using an orthotopic rat glioma model, confirming the antibody's ability to preferentially localize at the tumor site, with tumor/brain ratios superior to the ones observed with (18)F-fluorodeoxyglucose. These tumor-targeting properties, together with the strong immunohistochemical staining of human tumor sections, indicate that the G11 antibody may be used as a portable targeting moiety for the selective delivery of imaging and therapeutic agents to gliomas and lung tumors.

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CRRD Object Information
CRRD ID: 4889612
Created: 2010-12-07
Species: All species
Last Modified: 2010-12-07
Status: ACTIVE



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RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.