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Combinatorial SNARE complexes with VAMP7 or VAMP8 define different late endocytic fusion events.

Authors: Pryor, PR  Mullock, BM  Bright, NA  Lindsay, MR  Gray, SR  Richardson, SC  Stewart, A  James, DE  Piper, RC  Luzio, JP 
Citation: Pryor PR, etal., EMBO Rep. 2004 Jun;5(6):590-5. Epub 2004 May 7.
Pubmed: (View Article at PubMed) PMID:15133481
DOI: Full-text: DOI:10.1038/sj.embor.7400150

Both heterotypic and homotypic fusion events are required to deliver endocytosed macromolecules to lysosomes and remodel late endocytic organelles. A trans-SNARE complex consisting of Q-SNAREs syntaxin 7, Vti1b and syntaxin 8 and the R-SNARE VAMP8 has been shown by others to be responsible for homotypic fusion of late endosomes. Using antibody inhibition experiments in rat liver cell-free systems, we confirmed this result, but found that the same Q-SNAREs can combine with an alternative R-SNARE, namely VAMP7, for heterotypic fusion between late endosomes and lysosomes. Co-immunoprecipitation demonstrated separate syntaxin 7 complexes with either VAMP7 or VAMP8 in solubilized rat liver membranes. Additionally, overexpression of the N-terminal domain of VAMP7, in cultured fibroblastic cells, inhibited the mixing of a preloaded lysosomal content marker with a marker delivered to late endosomes. These data show that combinatorial interactions of SNAREs determine whether late endosomes undergo homotypic or heterotypic fusion events.


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CRRD Object Information
CRRD ID: 4892614
Created: 2011-02-25
Species: All species
Last Modified: 2011-02-25
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.