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Prolactin activates interferon regulatory factor-1 expression in normal lympho-hemopoietic cells.

Authors: Dogusan, Z  Book, ML  Verdood, P  Yu-Lee, LY  Hooghe-Peters, EL 
Citation: Dogusan Z, etal., Eur Cytokine Netw. 2000 Sep;11(3):435-42.
Pubmed: (View Article at PubMed) PMID:11022129

It has been proposed that prolactin (PRL) is a lympho-hemopoietic growth and differentiation factor. We show here by Western blotting that PRL-receptors (PRL-R) are expressed in normal rat bone marrow and spleen cells. We also show that PRL stimulates the phosphorylation of the PRL-R-associated Janus tyrosine kinase (JAK)-2 in rat bone marrow and spleen cells. This leads to the activation and subsequent binding of signal transducer and activator of transcription (Stat) 5b to an interferon regulatory factor-1 (IRF-1) gamma activation sequence (GAS) as visualized by electromobility shift assay. As shown after reverse transcription of mRNA by polymerase chain reaction, PRL, at physiological concentrations (0.01 microg/ml), stimulates the expression of the IRF-1 gene in these normal cells. PRL could thus affect several aspects of the immune response.


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CRRD Object Information
CRRD ID: 5128791
Created: 2011-03-16
Species: All species
Last Modified: 2011-03-16
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.