Adenosine A1 receptor agonists reduce hyperalgesia after spinal cord injury in rats.

Authors: Horiuchi, H  Ogata, T  Morino, T  Yamamoto, H 
Citation: Horiuchi H, etal., Spinal Cord. 2010 Sep;48(9):685-90. Epub 2010 Jan 12.
Pubmed: (View Article at PubMed) PMID:20065990
DOI: Full-text: DOI:10.1038/sc.2009.194

STUDY DESIGN: An in vivo study using a spinal cord compression model in rats. OBJECTIVES: To evaluate the effect of adenosine on thermal hyperalgesia after spinal cord injury (SCI).Summary of background data:After SCI, some patients suffer dysesthesia that is unresponsive to conventional treatments. We previously established a rat thoracic spinal cord mild-compression model by which we were able to induce thermal hyperalgesia in the hind limbs. METHODS: The thoracic spinal cord was compressed gently using a 20-g weight for 20 min. The withdrawal latency in response to thermal stimulation was monitored bilaterally in the hind limbs using Hargreaves' Plantar test apparatus. RESULTS: SCI-induced thermal hyperalgesia was mimicked by the intrathecal application of 8-cyclopentyl-1,3-dipropylxanthine (DPCPX), a selective adenosine A1 receptor antagonist. Hyperalgesia induced by SCI was significantly inhibited by the intrathecal application of 10-30 nmol chloro-adenosine (Cl-adenosine), a nonselective adenosine receptor agonist. The effect of Cl-adenosine (10 nmol) on hyperalgesia after SCI was blocked by the simultaneous application of DPCPX. Intrathecal application of R(-)N6-(2phenylisopropyl) adenosine (R-PIA; 10 nmol), a selective A1 receptor agonist, also inhibited SCI-induced hyperalgesia. In contrast, intrathecal application of CGS21680, a selective adenosine A2a receptor agonist, did not inhibit SCI-induced hyperalgesia. CONCLUSIONS: These results suggest that adenosine inhibits hyperalgesia through the stimulation of A1 receptors. Adenosine or adenosine A1 receptor agonists should be considered as candidates for new therapeutic methods for treating post-SCI dysesthesia.

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CRRD Object Information
CRRD ID: 5129094
Created: 2011-03-23
Species: All species
Last Modified: 2011-03-23
Status: ACTIVE



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RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.