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Elevated expression of adenosine A1 receptor in bronchial biopsy specimens from asthmatic subjects.

Authors: Brown, RA  Clarke, GW  Ledbetter, CL  Hurle, MJ  Denyer, JC  Simcock, DE  Coote, JE  Savage, TJ  Murdoch, RD  Page, CP  Spina, D  O'Connor, BJ 
Citation: Brown RA, etal., Eur Respir J. 2008 Feb;31(2):311-9. Epub 2007 Oct 24.
Pubmed: (View Article at PubMed) PMID:17959644
DOI: Full-text: DOI:10.1183/09031936.00003707

Asthmatics, unlike healthy subjects, experience bronchoconstriction in response to inhaled adenosine, and extracellular adenosine concentrations are elevated in the bronchoalveolar lavage fluid and exhaled breath condensate of asthmatic subjects. However, little is known about the location and expression of adenosine receptors in asthmatic airways. The aim of the present study was to investigate the distribution of adenosine A(1) receptors in bronchial biopsy specimens from mildly asthmatic steroid-naive subjects and then compare the degree of expression with that of healthy subjects. Biopsy sections were immunostained using an adenosine A(1) receptor antibody, the selectivity of which was validated in specific experiments. Image analysis was then performed in order to determine differences in immunostaining intensity. Immunostaining of biopsy sections from the asthmatic subjects revealed strong expression of the A(1) receptor, located predominantly in the bronchial epithelium and bronchial smooth muscle. In comparison, very weak immunostaining was observed in biopsy specimens obtained from healthy subjects. Image analysis revealed that the intensity of positive staining of the asthmatic bronchial epithelium and smooth muscle regions was significantly greater than that observed for the healthy epithelium and smooth muscle. In conclusion, the sensitivity of asthmatics to inhaled adenosine coupled with increased adenosine A(1) receptor expression implies that these receptors play a role in the pathophysiology of this disease.

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CRRD Object Information
CRRD ID: 5129097
Created: 2011-03-23
Species: All species
Last Modified: 2011-03-23
Status: ACTIVE



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