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Overexpression of nucleoside diphosphate kinases induces neurite outgrowth and their substitution to inactive forms leads to suppression of nerve growth factor- and dibutyryl cyclic AMP-induced effects in PC12D cells.

Authors: Ishijima, Y  Shimada, N  Fukuda, M  Miyazaki, H  Orlov, NY  Orlova, TG  Yamada, T  Kimura, N 
Citation: Ishijima Y, etal., FEBS Lett. 1999 Feb 19;445(1):155-9.
Pubmed: (View Article at PubMed) PMID:10069391

Whether nucleoside diphosphate kinase (NDPK) is involved in neuronal differentiation was investigated with special reference to its enzyme activity. Neurite outgrowth of PC12D cells induced by nerve growth factor or a cyclic AMP analog was suppressed to some extent when inactive NDPKs (the active site histidine 118 was replaced with alanine), not active forms, were transiently overexpressed. This suppression was more definite in their stably expressed clones. NDPKbeta-transfected clones and, to a lesser extent, NDPKalpha-transfected clones, but not inactive NDPK-transfected clones, extended neurites without differentiation inducers. These results imply that NDPKs may play a role by exerting their enzyme activity during differentiation of PC12 cells.


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CRRD Object Information
CRRD ID: 5132899
Created: 2011-06-07
Species: All species
Last Modified: 2011-06-07
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.