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Simultaneous expression of glutathione, thioredoxin-1, and their reductases in nerve transected hypoglossal motor neurons of rat.

Authors: Hama, I  Nakagomi, S  Konishi, H  Kiyama, H 
Citation: Hama I, etal., Brain Res. 2010 Jan 8;1306:1-7. Epub 2009 Oct 13.
Pubmed: (View Article at PubMed) PMID:19833109
DOI: Full-text: DOI:10.1016/j.brainres.2009.10.014

Anti-oxidative stress responses are crucial for the survival of nerve-injured motor neurons. Herein, we examined changes in expression of glutathione reductase (GSHr), thioredoxins (TRX1 and TRX2), and thioredoxin reductases (TRXr1 and TRXr2), important constituents of anti-oxidative pathways, following rat hypoglossal nerve transection. RT-PCR and in situ hybridization demonstrated that GSHr, TRX1, and TRXr1 mRNAs were significantly up-regulated during the first few weeks in nerve-injured motor neurons, while TRX2 and TRXr2 mRNAs were unchanged throughout 8 weeks after nerve transection. The up-regulation of GSH, GSHr, TRX1, and TRXr1 proteins in injured neurons was confirmed by immunohistochemical analysis. Western blotting also demonstrated up-regulation of GSHr, TRX1, and TRXr1 in injured neurons. These data suggest that the two major redox systems, GSH/GSHr and TRX1/TRXr1, are simultaneously activated in injured neurons, and likely provide protection of injured neurons against oxidative stress.


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CRRD Object Information
CRRD ID: 5133729
Created: 2011-06-28
Species: All species
Last Modified: 2011-06-28
Status: ACTIVE


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