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Vasoactive intestinal polypeptide, an erectile neurotransmitter, improves erectile function more significantly in castrated rats than in normal rats.

Authors: Zhang, MG  Shen, ZJ  Zhang, CM  Wu, W  Gao, PJ  Chen, SW  Zhou, WL 
Citation: Zhang MG, etal., BJU Int. 2011 Aug;108(3):440-6. doi: 10.1111/j.1464-410X.2010.09901.x. Epub 2010 Dec 16.
Pubmed: (View Article at PubMed) PMID:21166748
DOI: Full-text: DOI:10.1111/j.1464-410X.2010.09901.x

OBJECTIVE: * To investigate the regulatory role of androgen in VIP-mediated erectile effect. Androgen is essential for physiological erection. Vasoactive intestinal polypeptide (VIP) is an important erectile neurotransmitter. While previous studies demonstrated that VIP expression in the penis was androgen-independent, it remains controversial whether androgen has any effect on VIP-mediated erection. MATERIALS AND METHODS: * Male SD rats were divided into a control group, a castration group, and a castration-with-testosterone-replacement group. Four weeks later, each group was subdivided into low and high-dose VIP subgroups and subjected to intracavernous injection of 0.5 and 2 microg VIP, respectively. * Erectile function was tested by recording intracavernosal pressure (ICP) and mean arterial blood pressure (MAP) before and after VIP injection. * The expressions of the VIP-receptor (VPAC2), G-protein stimulatory and inhibitory alpha subunits (Gs-alpha, Gi-alpha), and PDE3A in rat corpus cavernosum (CC) was qualified by real-time PCR and Western blot analysis. RESULTS: * Castration reduced erectile function while testosterone restored it. VIP improved erectile function in a dose-dependent manner. * High-dose VIP significantly enhanced erectile function in castrated rats and there was no difference of ICP/MAP among three groups after injection of high-dose VIP. * Low-dose VIP also resulted in a higher improvement of erectile function in castrated rats, although the ICP/MAP was lower in these rats than in the other two groups. VPAC2 and Gs-alpha were up-regulated while Gi-alpha and PDE3A were down-regulated in CC of castrated rats. CONCLUSION: * VIP improves erectile function much more significantly in hypogonadal condition, mainly due to the higher expression of VPAC2, Gs-alpha, and lower expression of Gi-alpha and PDE3A in CC of castrated rats. Androgen may negatively regulate the erectile effect of VIP.

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CRRD Object Information
CRRD ID: 5685628
Created: 2012-01-12
Species: All species
Last Modified: 2012-01-12
Status: ACTIVE



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RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.