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Overexpression and hypomethylation of flap endonuclease 1 gene in breast and other cancers.

Authors: Singh, P  Yang, M  Dai, H  Yu, D  Huang, Q  Tan, W  Kernstine, KH  Lin, D  Shen, B 
Citation: Singh P, etal., Mol Cancer Res. 2008 Nov;6(11):1710-7.
Pubmed: (View Article at PubMed) PMID:19010819
DOI: Full-text: DOI:10.1158/1541-7786.MCR-08-0269

Flap endonuclease 1 (FEN1) is a structure-specific nuclease best known for its critical roles in Okazaki fragment maturation, DNA repair, and apoptosis-induced DNA fragmentation. Functional deficiencies in FEN1, in the forms of somatic mutations and polymorphisms, have recently been shown to lead to autoimmunity, chronic inflammation, and predisposition to and progression of cancer. To explore how FEN1 contributes to cancer progression, we examined FEN1 expression using 241 matched pairs of cancer and corresponding normal tissues on a gene expression profiling array and validated differential expression by quantitative real-time PCR and immunohistochemistry. Furthermore, we defined the minimum promoter of human FEN1 and examined the methylation statuses of the 5' region of the gene in paired breast cancer tissues. We show that FEN1 is significantly up-regulated in multiple cancers and the aberrant expression of FEN1 is associated with hypomethylation of the CpG island within the FEN1 promoter in tumor cells. The overexpression and promoter hypomethylation of FEN1 may serve as biomarkers for monitoring the progression of cancers.


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CRRD Object Information
CRRD ID: 6484520
Created: 2012-06-20
Species: All species
Last Modified: 2012-06-20
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.