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Matrix metalloproteinase-1 expression enhances tumorigenicity as well as tumor-related angiogenesis and is inversely associated with TIMP-4 expression in a model of glioblastoma.

Authors: Pullen, NA  Anand, M  Cooper, PS  Fillmore, HL 
Citation: Pullen NA, etal., J Neurooncol. 2012 Feb;106(3):461-71. Epub 2011 Aug 21.
Pubmed: (View Article at PubMed) PMID:21858729
DOI: Full-text: DOI:10.1007/s11060-011-0691-5

Herein we continue the study of matrix metalloproteinase-1 (MMP-1) with respect to glioblastoma multiforme (GBM) cell tumorigenicity and angiogenesis. A model of tumorigenicity with cells stably altered to over-express or knock-down MMP-1 revealed that it significantly increases tumor incidence and size. Organized endothelial growth in human umbilical vein endothelial cell (HUVEC)-GBM co-cultures was significantly increased in the presence of MMP-1. CD31 analysis of model tumors elucidated a substantial recruitment of endothelium in MMP-1 enhanced samples. Antibody arrays indicated an inverse expression of certain anti-angiogenic factors with respect to MMP-1, the most notable of which was a significant increase in tissue inhibitor of metalloproteinases-4 (TIMP-4) in the absence of MMP-1, as validated by immunoblot.


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CRRD Object Information
CRRD ID: 6771207
Created: 2012-07-18
Species: All species
Last Modified: 2012-07-18
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.