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[Adenosine alleviates hypoxia-induced rat right ventricular hypertrophy through the NHE-1/CaN signal pathway].

Authors: Lin, M  Huang, X  Tan, J  Wang, B 
Citation: Lin M, etal., Nan Fang Yi Ke Da Xue Xue Bao. 2012 May;32(5):734-7.
Pubmed: (View Article at PubMed) PMID:22588937

OBJECTIV: To investigate the effect of adenosine and its agonist on hypoxia-induced right ventricular hypertrophy (RVH) and explore the underlying mechanism. METHODS: Fifty-six rats were randomly divided into normoxia group, hypoxia group, and treated hypoxia groups (with different treatments with adenosine, A1 receptor agonist CPA, A2 receptor agonist NECA, CPA plus A1 receptor inhibitor DPCPX, or NECA plus A2B receptor inhibitor MRS1754). The rats except for those in normoxia group were exposed to normobaric chronic hypoxia (9.5%-10.5% oxygen) for 21 days, and the corresponding treatments were administered since the 7th day of hypoxia till day 21 via implantable capsule with a pressure pump. After the treatments, the right ventricles were then removed and weighed for evaluation of hypertrophy, and the expressions of NHE-1 and CnAbeta mRNA in the myocardial tissue were detected using RT-PCR. RESULTS: After a 21-day hypoxia, the rats showed significantly increased RV/(LV+S) ratio (0.369-/+0.033) and RV/BW ratio (0.75-/+0.095) compared to those in normoxia group (0.271-/+0.010 and 0.59-/+0.039, respectively; P<0.001), adenosine treatment group (0.281-/+0.022 and 0.65-/+0.077, respectively; P<0.001, P=0.025), hypoxia with CPA group (0.313-/+0.021and 0.66-/+0.067, respectively P<0.001), and hypoxia with NECA group(0.333-/+0.019, and 0.68-/+0.074, respectively P<0.001). The NHE-1 and CnAbeta mRNA levels in hypoxia group were significantly higher than those in normoxia group, adenosine treatment group, hypoxia with CPA group, and hypoxia with NECA group(P<0.001). CONCLUSION: Adenosine and its agonist can inhibit hypoxia-induced RVH in rats through the NHE-1/CaN signal pathway.


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CRRD Object Information
CRRD ID: 6771239
Created: 2012-07-24
Species: All species
Last Modified: 2012-07-24
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.