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Isolation and characterization of a cDNA for rat liver cysteine dioxygenase.

Authors: Hosokawa, Y  Matsumoto, A  Oka, J  Itakura, H  Yamaguchi, K 
Citation: Hosokawa Y, etal., Biochem Biophys Res Commun 1990 Apr 30;168(2):473-8.
Pubmed: (View Article at PubMed) PMID:2334417

Cysteine dioxygenase is a key enzyme of cysteine metabolism in mammals. The cDNA clones for rat liver cysteine dioxygenase were isolated by immunological screening and plaque hybridization from a rat liver cDNA library. The longest clone contained an insert of 1458 bp and encoded a polypeptide of 200 amino acids. The clone included the corresponding nucleotide sequence to amino acid sequences obtained from four lysyl endopeptidase-digested fragments of purified rat liver cysteine dioxygenase. The calculated molecular weight of rat liver cysteine dioxygenase was 23,025. Northern blot analysis revealed a single cysteine dioxygenase mRNA species of about 1.7 kb. A computer homology search indicated that this protein showed no homology with any known protein.


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CRRD Object Information
CRRD ID: 68765
Created: 2001-10-10
Species: All species
Last Modified: 2001-10-10
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.