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Identification and characterization of the high-affinity choline transporter.

Authors: Okuda, T  Haga, T  Kanai, Y  Endou, H  Ishihara, T  Katsura, I 
Citation: Okuda T, etal., Nat Neurosci 2000 Feb;3(2):120-5.
Pubmed: (View Article at PubMed) PMID:10649566
DOI: Full-text: DOI:10.1038/72059

In cholinergic neurons, high-affinity choline uptake in presynaptic terminals is the rate-limiting step in acetylcholine synthesis. Using information provided by the Caenorhabditis elegans Genome Project, we cloned a cDNA encoding the high-affinity choline transporter from C. elegans (cho-1). We subsequently used this clone to isolate the corresponding cDNA from rat (CHT1). CHT1 is not homologous to neurotransmitter transporters, but is homologous to members of the Na+-dependent glucose transporter family. Expression of CHT1 mRNA is restricted to cholinergic neurons. The characteristics of CHT1-mediated choline uptake essentially match those of high-affinity choline uptake in rat brain synaptosomes.


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CRRD Object Information
CRRD ID: 68853
Created: 2001-10-10
Species: All species
Last Modified: 2001-10-10
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.