Plakophilin-2 and the migration, differentiation and transformation of cells derived from the epicardium of neonatal rat hearts.

Authors: Matthes, SA  Taffet, S  Delmar, M 
Citation: Matthes SA, etal., Cell Commun Adhes. 2011 Aug;18(4):73-84. Epub 2011 Oct 10.
Pubmed: (View Article at PubMed) PMID:21985446
DOI: Full-text: DOI:10.3109/15419061.2011.621561

During development, epicardial cells act as progenitors for a large fraction of non-myocyte cardiac cells. Expression and function of molecules of the desmosome in the postnatal epicardium has not been studied. The objective of this study was to assess the expression of desmosomal molecules, and the functional importance of the desmosomal protein plakophilin-2 (PKP2), in epicardial and epicardium-derived cells. Epicardial explants were obtained from neonatal rat hearts. Presence of mechanical junction proteins was assessed by immunocytochemistry. Explants after PKP2 knockdown showed increased abundance of alpha smooth muscle actin-positive cells, increased abundance of lipid markers, enhanced cell migration velocity and increased abundance of a marker of cell proliferation. We conclude that a population of non-excitable, cardiac-resident cells express desmosomal molecules and, in vitro, show functional properties (including lipid accumulation) that depend on PKP2 expression. The possible relevance of our data to the pathophysiology of arrhythmogenic right ventricular cardiomyopathy, is discussed.


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CRRD Object Information
CRRD ID: 6892694
Created: 2012-08-13
Species: All species
Last Modified: 2012-08-13
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.