RGD Reference Report - Recruitment of an alternatively spliced form of synaptojanin 2 to mitochondria by the interaction with the PDZ domain of a mitochondrial outer membrane protein. - Chinchilla Research Resource Database
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Recruitment of an alternatively spliced form of synaptojanin 2 to mitochondria by the interaction with the PDZ domain of a mitochondrial outer membrane protein.

Authors: Nemoto, Y  De Camilli, P 
Citation: Nemoto Y and De Camilli P, EMBO J 1999 Jun 1;18(11):2991-3006.
CRRD ID: 69391
Pubmed: (View Article at PubMed) PMID:10357812
DOI: Full-text: DOI:10.1093/emboj/18.11.2991

Synaptojanin 1 is an inositol 5'-phosphatase highly enriched in nerve terminals with a putative role in recycling of synaptic vesicles. We have previously described synaptojanin 2, which is more broadly expressed as multiple alternatively spliced forms. Here we have identified and characterized a novel mitochondrial outer membrane protein, OMP25, with a single PDZ domain that specifically binds to a unique motif in the C-terminus of synaptojanin 2A. This motif is encoded by the exon sequence specific to synaptojanin 2A. OMP25 mRNA is widely expressed in rat tissues. OMP25 is localized to the mitochondrial outer membrane via the C-terminal transmembrane region, with the PDZ domain facing the cytoplasm. Overexpression of OMP25 results in perinuclear clustering of mitochondria in transfected cells. This effect is mimicked by enforced expression of synaptojanin 2A on the mitochondrial outer membrane, but not by the synaptojanin 2A mutants lacking the inositol 5'-phosphatase domain. Our findings provide evidence that OMP25 mediates recruitment of synaptojanin 2A to mitochondria and that modulation of inositol phospholipids by synaptojanin 2A may play a role in maintenance of the intracellular distribution of mitochondria.

Annotation

Gene Ontology Annotations    

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Cellular Component

Molecular Function

Objects Annotated

Objects referenced in this article
0 Synj2 synaptojanin 2 All species
0 Synj2_v3 synaptojanin 2, variant 3 All species
0 Synj2bp synaptojanin 2 binding protein All species

Additional Information