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Molecular cloning and expression of a rat cDNA encoding 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase.

Authors: Akira, T  Komatsu, M  Nango, R  Tomooka, A  Konaka, K  Yamauchi, M  Kitamura, Y  Nomura, S  Tsukamoto, I 
Citation: Akira T, etal., Gene 1997 Sep 15;197(1-2):289-93.
Pubmed: (View Article at PubMed) PMID:9332377

The cDNA of a 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (AICARFT/IMPCHase) was isolated from rat liver RNA by reverse transcription and the polymerase chain reaction (PCR). The rat AICARFT/IMPCHase cDNA included 1928 bp containing a coding region of 1779 bp for a 592-amino acid polypeptide (Mr = 64 200). Rat and human AICARFT/IMPCHase cDNAs show 84 and 91% homology at the nucleotide and amino acid sequence level, respectively. The protein produced by the rat cDNA using pET-expression system catalysed the penultimate and final steps of de novo purine biosynthesis. Northern analysis identified a 2.8-kb AICARFT/IMPCHase mRNA and the level of the AICARFT/IMPCHase transcripts increased markedly at 24 h after partial (70%) hepatectomy.

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CRRD Object Information
CRRD ID: 70804
Created: 2002-06-18
Species: All species
Last Modified: 2002-06-18
Status: ACTIVE



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