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Extracellular calcium modulates in vitro bone marrow-derived Flk-1+ CD34+ progenitor cell chemotaxis and differentiation through a calcium-sensing receptor.

Authors: Aguirre, A  Gonzalez, A  Planell, JA  Engel, E 
Citation: Aguirre A, etal., Biochem Biophys Res Commun. 2010 Feb 26;393(1):156-61. doi: 10.1016/j.bbrc.2010.01.109. Epub 2010 Feb 1.
Pubmed: (View Article at PubMed) PMID:20117086
DOI: Full-text: DOI:10.1016/j.bbrc.2010.01.109

Angiogenesis is a complex process regulated by many cell types and a large variety of biochemical signals such as growth factors, transcription factors, oxygen and nutrient diffusion among others. In the present study, we found out that Flk-1(+) CD34(+) progenitor cells (bone marrow resident cells with an important role in angiogenesis) were responsive to changes in extracellular calcium concentration through a membrane bound, G-protein-coupled receptor sensitive to calcium ions related to the calcium-sensing receptor (CaSR). Calcium was able to induce progenitor cell migration in Boyden chamber experiments and tubulogenesis in Matrigel assays. Addition of anti-CaSR antibodies completely blocked the effect, while CaSR agonist Mg(2+) produced a similar response to that of calcium. Real time RT-PCR for a wide array of angiogenesis-related genes showed increased expression of endothelial markers and signaling pathways involved in angiogenesis. These results suggest calcium could be a physiological modulator of the bone marrow progenitor cell-mediated angiogenic response.

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CRRD Object Information
CRRD ID: 7206840
Created: 2013-01-15
Species: All species
Last Modified: 2013-01-15
Status: ACTIVE



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