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The effect of 2,3,4',5-tetrahydroxystilbene-2-0-beta-D glucoside on neointima formation in a rat artery balloon injury model and its possible mechanisms.

Authors: Xu, XL  Ling, DY  Zhu, QY  Fan, WJ  Zhang, W 
Citation: Xu XL, etal., Eur J Pharmacol. 2013 Jan 5;698(1-3):370-8. doi: 10.1016/j.ejphar.2012.11.019. Epub 2012 Nov 21.
Pubmed: (View Article at PubMed) PMID:23178522
DOI: Full-text: DOI:10.1016/j.ejphar.2012.11.019

2,3,4',5-tetrahydroxystilbene-2-0-beta-D glucoside (TSG) has been recognized to suppress the proliferation of vascular smooth muscle cells (VSMCs). The aim of the present study was to determine whether TSG inhibits neointimal hyperplasia in a rat carotid arterial balloon injury model. Balloon injury was induced in the left common carotid artery of rats. TSG (30, 60, 120 mg/kg/day) was treated from 3 days prior to, until 14 days after the induction of balloon injury. The ratio of intima-to-media was significantly reduced in the TSG-treated rats at 14 days after the induction of injury, which was associated with reduced expressions of proliferating cell nuclear antigen (PCNA), alpha-smooth muscle actin (alpha-SMA) and platelet-derived growth factor-BB (PDGF-BB), as markers of VSMCs proliferation and migration. Additionally, TSG significantly inhibited PDGF-BB induced cell migration in cultured VSMCs. Furthermore, we explored the underlying mechanisms for such effects of TSG. The result showed that TSG markedly reduced balloon injury-induced AKT, extracellular signal-regulated kinase (ERK1/2) and nuclear factor kappaB (NF-kappaB) activation as well as mRNA expressions of c-myc, c-fos and c-jun, which is important signal pathway for VSMCs proliferation. And in both vivo and vitro model, TSG markedly regulated matrix metalloproteinase-2, 9 expressions and collagen I, III expressions, which are key factors in extracellular matrix for VSMCs migration. These results suggest that the anti-proliferative and anti-migrative effects of TSG on VSMCs could help to explain the beneficial effects of TSG on neointima hyperplasia induced by balloon injury.


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CRRD Object Information
CRRD ID: 7207861
Created: 2013-02-11
Species: All species
Last Modified: 2013-02-11
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.