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Proteomics reveal a concerted upregulation of methionine metabolic pathway enzymes, and downregulation of carbonic anhydrase-III, in betaine supplemented ethanol-fed rats.

Authors: Kharbanda, KK  Vigneswara, V  McVicker, BL  Newlaczyl, AU  Bailey, K  Tuma, D  Ray, DE  Carter, WG 
Citation: Kharbanda KK, etal., Biochem Biophys Res Commun. 2009 Apr 17;381(4):523-7. doi: 10.1016/j.bbrc.2009.02.082. Epub 2009 Feb 23.
Pubmed: (View Article at PubMed) PMID:19239903
DOI: Full-text: DOI:10.1016/j.bbrc.2009.02.082

We employed a proteomic profiling strategy to examine the effects of ethanol and betaine diet supplementation on major liver protein level changes. Male Wistar rats were fed control, ethanol or betaine supplemented diets for 4 weeks. Livers were removed and liver cytosolic proteins resolved by one-dimensional and two-dimensional separation techniques. Significant upregulation of betaine homocysteine methyltransferase-1, methionine adenosyl transferase-1, and glycine N-methyltransferase were the most visually prominent protein changes observed in livers of rats fed the betaine supplemented ethanol diet. We hypothesise that this concerted upregulation of these methionine metabolic pathway enzymes is the protective mechanism by which betaine restores a normal metabolic ratio of liver S-adenosylmethionine to S-adenosylhomocysteine. Ethanol also induced significant downregulation of carbonic anhydrase-III protein levels which was not restored by betaine supplementation. Carbonic anhydrase-III can function to resist oxidative stress, and we therefore hypothesise that carbonic anhydrase-III protein levels compromised by ethanol consumption, contribute to ethanol-induced redox stress.


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CRRD Object Information
CRRD ID: 7242903
Created: 2013-04-24
Species: All species
Last Modified: 2013-04-24
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.