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Iron metabolism and its relationship to anemia and immune system in Trypanosoma evansi infected rats.

Authors: Da Silva, CB  Wolkmer, P  Paim, FC  Da Silva, AS  Siqueira, LC  De Souza, CL  Franca, RT  Dornelles, GL  Medeiros Frescura Duarte, MM  Monteiro, SG  Mazzanti, CM  Dos Anjos Lopes, ST 
Citation: da Silva CB, etal., Exp Parasitol. 2013 Mar;133(3):357-64. doi: 10.1016/j.exppara.2012.12.010. Epub 2012 Dec 25.
Pubmed: (View Article at PubMed) PMID:23270806
DOI: Full-text: DOI:10.1016/j.exppara.2012.12.010

The aim of this study was to evaluate biochemical parameters of iron metabolism in rats experimentally infected with Trypanosoma evansi. To this end, 20 rats (Wistar) were intraperitoneally inoculated with blood containing trypomastigotes 10(6) (Group T) and 12 animals were used as negative control (Group C) and received saline (0.2 mL) through same route. Blood samples were collected by cardiac puncture on day 5 (C5, T5) and 30 (C30, T30) post-inoculation (pi) to perform complete blood count and determination of serum iron, transferrin, ferritin, total and latent iron fixation capacity, transferrin saturation and prohepcidin concentration. Also, bone marrow samples were collected, to perform Pearls staining reaction. Levels of iron, total and latent iron binding capacity and prohepcidin concentration were lower (P<0.05) in infected rats (T5 and T30 groups) compared to controls. On the other hand, levels of transferrin and ferritin were higher when compared to controls (P<0.05). The transferrin saturation increased on day 5 pi, but decreased on day 30 pi. The Pearls reaction showed a higher accumulation of iron in the bone marrow of infected animals in day 5 pi (P<0.01). Infection with T. evansi in rats caused anemia and changes in iron metabolism associated to the peaks of parasitemia. These results suggest that changes in iron metabolism may be related to the host immune response to infection and anemic status of infected animals.

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CRRD Object Information
CRRD ID: 7244377
Created: 2013-06-04
Species: All species
Last Modified: 2013-06-04
Status: ACTIVE



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RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.