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The cloning of a rat peptidoglycan recognition protein (PGRP) and its induction in brain by sleep deprivation.

Authors: Rehman, A  Taishi, P  Fang, J  Majde, JA  Krueger, JM 
Citation: Rehman A, etal., Cytokine 2001 Jan 7;13(1):8-17.
Pubmed: (View Article at PubMed) PMID:11145837
DOI: Full-text: DOI:10.1006/cyto.2000.0800

Peptidoglycan recognition protein (PGRP) binds to peptidoglycan (PG) or live bacteria and is upregulated by PG. PGRP is a ubiquitous protein involved in innate immunity. Tag7, a novel cytokine, is also induced by bacterial products; tag7 is apoptotic to murine L929 cells in a NF-kappaB-independent manner. Both of these genes are expressed in brain, lymphatic and haematopoietic tissues. We provide evidence that murine PGRP and tag7 encode identical transcripts and have structural relationships to lysozymes. Further, we have cloned the cDNA of rat PGRP and analyzed its expression in brains of sleep-deprived and control rats. The mRNA levels of PGRP/tag7 were measured by RT-PCR and compared to the housekeeping gene porphobilinogen deaminase (PBD). PGRP was constitutively expressed in rat brain. PGRP mRNA was increased by 43% and 17% in the brainstem and hypothalamus, respectively, in sleep-deprived rats compared to controls. The upregulation of PGRP expression by sleep deprivation suggests a role for PGRP in a homeostatic regulation of sleep.

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CRRD Object Information
CRRD ID: 724610
Created: 2003-10-10
Species: All species
Last Modified: 2003-10-10
Status: ACTIVE



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RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.