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Production of knockout rats using ENU mutagenesis and a yeast-based screening assay.

Authors: Zan, Y  Haag, JD  Chen, KS  Shepel, LA  Wigington, D  Wang, YR  Hu, R  Lopez-Guajardo, CC  Brose, HL  Porter, KI  Leonard, RA  Hitt, AA  Schommer, SL  Elegbede, AF  Gould, MN 
Citation: Zan Y, etal., Nat Biotechnol 2003 Jun;21(6):645-51.
Pubmed: (View Article at PubMed) PMID:12754522
DOI: Full-text: DOI:10.1038/nbt830

The rat is a widely used model in biomedical research and is often the preferred rodent model in many areas of physiological and pathobiological research. Although many genetic tools are available for the rat, methods to produce gene-disrupted knockout rats are greatly needed. In this study, we developed protocols for creating N-ethyl-N-nitrosourea (ENU)-induced germline mutations in several rat strains. F1 preweanling pups from mutagenized Sprague Dawley (SD) male rats were then screened for functional mutations in Brca1 and Brca2 using a yeast gap-repair, ADE2-reporter truncation assay. We produced knockout rats for each of these two breast cancer suppressor genes.


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CRRD Object Information
CRRD ID: 727990
Created: 2003-11-06
Species: All species
Last Modified: 2003-11-06
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.