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GABAA receptor beta subunit heterogeneity: functional expression of cloned cDNAs.

Authors: Ymer, S  Schofield, PR  Draguhn, A  Werner, P  Kohler, M  Seeburg, PH 
Citation: Ymer S, etal., EMBO J 1989 Jun;8(6):1665-70.
Pubmed: (View Article at PubMed) PMID:2548852

Cloned cDNAs encoding two new beta subunits of the rat and bovine GABAA receptor have been isolated using a degenerate oligonucleotide probe based on a highly conserved peptide sequence in the second transmembrane domain of GABAA receptor subunits. The beta 2 and beta 3 subunits share approximately 72% sequence identity with the previously characterized beta 1 polypeptide. Northern analysis showed that both beta 2 and beta 3 mRNAs are more abundant in the brain than beta 1 mRNA. All three beta subunit encoding cDNAs were also identified in a library constructed from adrenal medulla RNA. Each beta subunit, when co-expressed in Xenopus oocytes with an alpha subunit, forms functional GABAA receptors. These results, together with the known alpha subunit heterogeneity, suggest that a variety of related but functionally distinct GABAA receptor subtypes are generated by different subunit combinations.


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CRRD Object Information
CRRD ID: 728451
Created: 2003-11-19
Species: All species
Last Modified: 2004-05-25
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.