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Isolation of a cDNA encoding the rat liver S-adenosylmethionine synthetase.

Authors: Horikawa, S  Ishikawa, M  Ozasa, H  Tsukada, K 
Citation: Horikawa S, etal., Eur J Biochem 1989 Oct 1;184(3):497-501.
Pubmed: (View Article at PubMed) PMID:2806235

We have isolated cDNA clones encoding the rat liver S-adenosylmethionine synthetase by means of immunological screening from a phage lambda gt 11 expression library containing cDNA synthesized from adult rat liver poly(A)-RNA. The amino acid sequence deduced from the cDNA indicates that the rat liver enzyme for this protein contains 397 amino acid residues and has a molecular mass of 43697 Da. The deduced amino acid sequence of rat liver S-adenosylmethionine synthetase was 68% similar to those of yeast S-adenosylmethionine synthetases encoded by two unlinked genes SAM1 and SAM2. The rat liver S-adenosylmethionine synthetase also shows 52% similarity with the deduced amino acid sequence of the MetK gene encoding the S-adenosylmethionine synthetase in Escherichia coli.


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CRRD Object Information
CRRD ID: 728958
Created: 2003-11-25
Species: All species
Last Modified: 2003-11-25
Status: ACTIVE


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