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Analysis of the organization and nucleotide sequence of the chromosomal gene for the beta-subunit of rat thyrotropin.

Authors: Croyle, ML  Bhattacharya, A  Gordon, DF  Maurer, RA 
Citation: Croyle ML, etal., DNA 1986 Aug;5(4):299-304.
Pubmed: (View Article at PubMed) PMID:3017658

The gene for the beta-subunit of rat thyrotropin has been isolated from a library of rat DNA fragments cloned in bacteriophage lambda. The complete nucleotide sequence of the gene has been determined including a portion of 5'- and 3'-flanking regions. The rat TSH-beta gene contains approximately 4879 nucleotides which ultimately lead to the production of a mRNA of about 554 nucleotides, exclusive of the 3' poly(A) tract. The first exon which represents only 5' untranslated sequences of the mRNA, is separated from the second exon by a very large, 3.9-kbp intervening sequence. The second and third exons are separated by a small, 377-bp intervening sequence. Southern blot analysis of total genomic DNA demonstrated that the rat genome contains sequences similar to the cloned gene, suggesting that no rearrangements occurred during the cloning process.

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CRRD Object Information
CRRD ID: 729568
Created: 2003-11-26
Species: All species
Last Modified: 2004-05-25
Status: ACTIVE



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RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.